Journal: Open Veterinary Journal

Article Title: Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease

doi: 10.5455/OVJ.2024.v14.i5.12

Figure Lengend Snippet: Trilineage differentiation potency. Canine LCPD-affected femoral head-derived adherent cells (A-C) and BMMSCs (D-F) differentiated into adipocytes (A and D), osteoblasts (B and E), and chondrocytes (C and F). (A and D): Sudan III staining, (B and E): Alizarin Red staining, and (C and F): Alcian blue staining. Scale bars = 100 μm or 1 mm.

Article Snippet: To induce differentiation into chondrocytes, 5 × 10 5 cells were placed in 15 ml polypropylene tubes, centrifuged to form pellets, and cultured with canine chondrocyte differentiation medium (Cell Applications, Inc.) for 3 weeks.

Techniques: Derivative Assay, Staining

Journal: PLoS ONE

Article Title: Interleukin-6 Synthesis in Human Chondrocytes Is Regulated via the Antagonistic Actions of Prostaglandin (PG)E 2 and 15-deoxy-Δ 12,14 -PGJ 2

doi: 10.1371/journal.pone.0027630

Figure Lengend Snippet: T/C-28a2 chondrocytes were treated with either PGD 2 (A) or 15d-PGJ 2 (B) for 48 h, or PGE 2 (C) for 2 h. TLR4, caveolin-1 and IL-6 protein (upper) and mRNA (lower) expression was determined by Western blotting or qRT-PCR, respectively. ß-actin and GAPDH served as internal controls in immunoblotting and qRT-PCR, respectively. The Western blots are representative of three independent experiments, all revealing similar results. Data represent the mean ± S.E. of 3 independent qRT-PCR experiments. * and ▴, p <0.05 with respect to the corresponding vehicle control.

Article Snippet: Human primary articular chondrocytes (Cell Applications, Inc) or T/C-28a2 chondrocytic cells (T/C-28a2 chondrocytic cells were kindly provided by Dr. Goldring at Harvard Medical School, Boston, MA, USA) were seeded on 6-cm tissue culture dishes (10 6 cells per dish) in human chondrocyte growth medium (Cell Applications, Inc) or in DMEM/F12 medium supplemented with 10% FBS, respectively , , , , , .

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Control

Journal: PLoS ONE

Article Title: Interleukin-6 Synthesis in Human Chondrocytes Is Regulated via the Antagonistic Actions of Prostaglandin (PG)E 2 and 15-deoxy-Δ 12,14 -PGJ 2

doi: 10.1371/journal.pone.0027630

Figure Lengend Snippet: PGE 2 stimulates TLR4 synthesis, which is in turn responsible for the activation of the ERK1/2, PI3K/Akt and PKA/CREB pathways that phosphorylate the NF-κB p65 subunit leading to NF-κB activation. Binding of the activated NF-κB p65 subunit to IL-6 promoter induces IL-6 synthesis in human chondrocytes. PGD 2 or 15d-PGJ 2 concurrently downregulates TLR4 and upregulates caveolin-1, which in turn inhibit the PGE 2 -dependent ERK1/2, PI3-K and PKA activation, and ultimately with NF-κB-dependent IL-6 synthesis in chondrocytes.

Article Snippet: Human primary articular chondrocytes (Cell Applications, Inc) or T/C-28a2 chondrocytic cells (T/C-28a2 chondrocytic cells were kindly provided by Dr. Goldring at Harvard Medical School, Boston, MA, USA) were seeded on 6-cm tissue culture dishes (10 6 cells per dish) in human chondrocyte growth medium (Cell Applications, Inc) or in DMEM/F12 medium supplemented with 10% FBS, respectively , , , , , .

Techniques: Activation Assay, Binding Assay